Overview
Plant virus and sub-viral pathogens pose severe constraints to the production of wide range of economically important crops worldwide.\xa0 The crops raised both through true seed and vegetative propagated materials are affected with number of virus and virus-like diseases. The virus may enter into plants through seed planting materials or by vectors.\xa0 Once the virus is in the field, it multiplies and spreads following definite patterns depending upon the nature of the vector and agro-meteorological conditions.\xa0 Disease free crops and plants are great economic and social importance in feeding the world's population. Detection of virus and sub-viral agents at initial stages of infection is critical to reduce economic losses.\xa0 For nearly two decades, ELISA and its variants played a major role in large scale virus testing and also in the production of virus-free planting materials.
