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Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species-including mice, sheep, cattle, and non-mammals-is considered as well.   The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine - Dr John Gurdon; the cloning of the first mammal from a somatic cell - Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual - Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell - Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas.

Principles of Cloning

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PRINCIPLES OF CLONING 2

Duration

Duration Unit Of Measure

Transaction Type

Front Cover.
Half Title Page.
Title Page.
Copyright Page.
Editorial Advisory Board.
Contents.
Foreword to the Second Edition.
Foreword to the First Edition.
Preface to the First Edition.
In Remembrance of Keith Henry Stockman Campbell (23 May 1954&#x2013;5 October 2012).
List of Contributors.
1: Basics.
2: Artificial Activation of Mammalian Oocytes for Cloning: Present Status and Future Perspectives.
3: Epigenetic Reprogramming of Somatic Nuclei via Cell Fusion.
4: Nuclear Origins and Clone Phenotype: What Cloning Tells Us about Embryonic Development.
5: Methods.
6: Micromanipulation Techniques for Cloning.
7: Nuclear Transfer with Germ Cells: Germ Cell Cloning Contributes to Current Understanding of Genomic Imprinting in Mammals.
8: Culture of Viable Mammalian Embryos In Vitro.
9: Genetic and Phenotypic Similarity Among Members of Mammalian Clonal Sets.
10: Advances in the Generation of Transgenic Domestic Species via Somatic Cell Nuclear Transfer.
11: Pregnancy and Neonatal Care of SCNT Animals.
12: Donor Cell Type and Cloning Efficiency in Mammals.
13: Enhancing SCNT with Chromatin Remodeling Agents.
14: Cell Cycle Regulation in Cloning.
15: Clone-Specific X-Linked Gene Repression Caused by Ectopic Xist Transcripts from the Active X Chromosome.
16: Cloning by Species.
17: Cloning of Amphibia.
18: Cloning of Medaka Fish.
19: Somatic Cell Nuclear Transfer in Zebrafish.
20: Cloning of Mice.
21: Cloning of Rabbits.
22: Cloning Pigs by Somatic Cell Nuclear Transfer.
23: A Historical Perspective of the Cloning of Cattle.
24: Cloning of Canines.
25: Cloning of Equines.
26: Cloning Primates.
27: Applications.
28: Nuclear Transfer for Stem Cells.
29: Current Research and Commercial Applications of Cloning Technology.
30: Transgenic Cloned Goats and the Production of Recombinant Therapeutic Proteins.
31: Commercializing Genetically Engineered Cloned Cattle.
32: Cloning Endangered Species.
33: Complementary Technologies.
34: Genome Editing in Somatic Cells Using Zinc Finger Nucleases and Transcription Activator-Like Effector Nucleases.
35: Targeted Chromosome Elimination from ES-Somatic Hybrid Cells.
36: SCNT as a Tool to Answer Biological Questions.
37: How Cell Division Facilitates Nuclear Reprogramming.
38: Proteomic Approach to the Reprogramming Machinery of the Mouse Oocyte.
39: Biological Age of Cloned Animals.
40: Mitochondrial DNA.
41: Interspecies Somatic Cell Nuclear Transfer.
42: Epigenetics of Cloned Livestock Embryos and Offspring.
43: Comparing SCNT-Derived ESCs and iPSCs.
44: Role of iPSC-Producing Factors in Pre-Implantation Embryos.
45: Ethical and Legal Affairs.
46: Genome Exchange in Human Oocytes.
47: Ethical Implications of Reproductive Cloning.
48: An Overview of the Regulatory Considerations for Animal Cloning.
Author Index.
Subject Index.

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Principles of Cloning