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One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors.  Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening.

Fluorescence-Based Biosensors

POST SECONDARY FOUR YEAR

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FLUORESCENCE-BASED BIOSENSORS

Duration

Duration Unit Of Measure

Transaction Type

Front Cover.
Half Title.
Title Page.
copyright page.
Contents.
Contributors.
Preface.
Introduction.
1: The Chemistry of Small-Molecule Fluorogenic Probes.
2: Fluorescent Environment-Sensitive Dyes as Reporters of Biomolecular Interactions.
3: Rational Design of Fluorophores for In Vivo Applications.
4: Fluorescence Technologies for Monitoring Interactions Between Biological Molecules In Vitro.
5: From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells.
6: Fluorescent Sensors of Protein Kinases: From Basics to Biomedical Applications.
7: Time-Resolved F&#x00F6;rster Resonance Energy Transfer-Based Technologies to Investigate G Protein-Coupled Receptor Machinery: High-Throughput Screening Assays and Future Development.
8: Fluorescent Protein-Based Biosensors and Their Clinical Applications.
9: Fluorescent Macromolecular Sensors of Enzymatic Activity for In Vivo Imaging.
10: Fluorescent Proteins as Visible In Vivo Sensors.
Index.
Other Backmatter.

Fluorescence-Based Biosensors.css-1p8rvzy{position:absolute;padding-left:8px;}

Fluorescence-Based Biosensors