zone1

footer-menu-component

Would you like to go to the Gale {{RECOMMENDED_COUNTRY}} site instead?

It looks like you're visiting from {{DETECTED_COUNTRY}}.

main

footer-menu

Academic Institutions

K-12

Public Libraries

Government and Special Libraries

Statewide Programs

Institutions

Databases

Primary Sources

Digital Humanities Tools

eLearning

Schools/K-12 Curriculum

Books

Large Print Books by Thorndike Press

Products

Find Your Rep

Product Support

Technical Support

Customer Support

Admin Tools

Accessibility Policy

Support

About Us

Blogs

Press Center

Events

News

Careers

Partnerships

Contact Us

Our Company

Account Information

bundle-connector

footer-legal-menu-component

container

secondary-primary-menu

mega-menu

zone3

products

Marketing materials, training documents, discovery services

cengage:card

FIND YOUR SALES REPRESENTATIVE

cengage:column

Get marketing materials, training documents, discovery services.

PRODUCT SUPPORT

CUSTOMER SUPPORT

Report technical issues, errors, check system status

TECHNICAL SUPPORT

Manage product preferences, generate usage reports, simplify administrative tasks.

ADMIN TOOLS

support

footer-social-menu-component

cengage:galethorndikeseo

secondary-storefront-menu

International

North America

China

Japan

Latin America

Brazil

Region

Set your region:

header

footer-social-menu

Sign Up for Emails

CONNECT WITH GALE

Stay up-to-date with the latest Gale news, exclusive offers, and more.

facebook

linkedin

instagram

youtube

SOCIAL ICONS

Browse by Institution Type

Browse by Product Type

Request a Trial

secondary-account-menu

gale-footer

footer

FIND YOUR REP

support-order-2

header-component

single-column

footer-legal-menu

Terms of use

Privacy

%1 products have been added to your cart but %2 have been backordered and will be fulfilled when stock is available

Product on backorder

Current quantity limit is [maxProductQuantity], please, navigate to the cart page and change quantity of product

You will be able to resubmit promo in ${countdown} seconds. test

Please note that the taxes displayed on our website are estimated and subject to adjustment. The actual tax charges will be determined and settled during the final invoicing of your order

product-detail-component

zone2

You may already have access to Gale content through your institution.

STUDENTS AND RESEARCHERS

support-order-1

Profile

Orders

Wish List

Fast Order Entry

My Account

config

How Free Cationic Polymer Chains Promote Gene Transfection

ST: HOW FREE CATIONIC POLYMER CHAINS PRMT GENE TRNSFCTN

Chemistry

Mathematics & Science

Gt Product Group

Binding Format

Marketing Imprint Name

B2B GT

CL Carton Count

Imprint Name

B2B & B2C

In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted "proton sponge" model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

POST SECONDARY FOUR YEAR

COLFR

COLJR

COLSO

COLSR

Duration

Duration Unit Of Measure

Transaction Type

Front Cover.
Other Frontmatter.
Other Frontmatter.
Title Page.
Copyright Page.
Other Frontmatter.
Supervisor&#x0027;s Foreword.
Acknowledgments.
Contents.
Abstract.
Symbols and Abbreviations.
1: Introduction and Background.
2: Revisiting Complexation between DNA and Polyethylenimine: The Effect of Uncomplexed Chains Free in the Solution Mixture on Gene Transfection.
3: Revisiting Complexation between DNA and Polyethylenimine: The Effect of Length of Free Polycationic Chains on Gene Transfection.
4: Quantitative Comparison of Endocytosis and Intracellular Trafficking of DNA/Polymer Complexes in the Absence/Presence of Free Polycationic Chains.

How Free Cationic Polymer Chains Promote Gene Transfection.css-1p8rvzy{position:absolute;padding-left:8px;}

How Free Cationic Polymer Chains Promote Gene Transfection