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Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo

ST: BMLCLR IMGG HGH SPTL & TEMPRL RSLTN VITRO & IN VIVO

Engineering

Mathematics & Science

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B2B & B2C

As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.

POST SECONDARY FOUR YEAR

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Front Cover.
Other Frontmatter.
Other Frontmatter.
Title Page.
Copyright Page.
Other Frontmatter.
Supervisor&#x0027;s Foreword.
Abstract.
Acknowledgments.
Contents.
Abbreviations.
1: Introduction.
2: Biomolecules and Microscopy.
3: Materials and Methods.
4: Experimental Methods.
5: Imaging Methods.
6: Experimental Results.
7: Towards an Atomistic Structure of a De Novo Designed Peptide Fibre.
8: Developing Novel Probes for Correlative Light Electron Microscopy.
Concluding Remarks.
Amino Acid Structures.
Vectors, Primers and Recipes.

Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo.css-1p8rvzy{position:absolute;padding-left:8px;}

Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo